Last Updated April 2012 Copyright @ Prof. Dr. Hany Marei, Faculty of Veterinary Medicine, Mansoura University, Egypt http://www.vetmansoura.com/Pathology/Index/Index.html Neural Stem Cells  NSC 1: The NSCs were isolated from human olfactory bulb and were expended in vitro. They were transfected with green fluorescent protein (GFP), and nerve growth factor (NGF) genes.      Spinal Cord Injury  SCI Rat Model 2: Under aseptic conditions, a T7-T8 laminectomy was performed. The dorsal two-third of the SC is transected with microscissors.  Rats (n=20) were rcieve transplants of NSCs (100 000 cells in 2 ul). Parkinson’s Model PD Rat Model 3: 6OHDA PD model (n=20) were performed by stereotaxic injection of 6OHPD in the striatum. The PD models were assessted by amphetamine rotation test.  Alzheimer’s Model AD model 4: Iboteinic acid AD model (n=20) were performed by stereotaxic injection ofIboteinic acid in the hippocampus. The AD models were assessted by Morris Water maze test.   The wider objectives of the present project are: Establish stem cell lab in Mansoura University. TOT for PI about the most recent sophisticated genetic and molecular biological approaches. To isolate NS/P cells from olfactory bulb. To optimize the culture conditions, methodology and techniques for OB-derived NS/P cells. To identify genes responsible for differentiation of OB-derived NS/P cells. To demonstrate the gene expression profiles for subtypes of OB-derived NS/P cells. Test the efficiency of cell-based therapy with OB-derived NS/P cells for treatment of spinal cord and CNS injuries. To test the efficiency of cell-based therapy with OB-derived NS/P cells for treatment of Parkinson's and Alzheimer's.